TY - JOUR ID - 39074 TI - Chemiluminescence Determination of Hydroxyzine and its Metabolite Cetirizine JO - Analytical and Bioanalytical Chemistry Research JA - ABCR LA - en SN - AU - Mokhtari, Ali AU - Benam, Mahboobeh AU - Keyvanfard, Mohsen AU - Ghazaeian, Mehrgan AD - Department of Chemistry, Golestan University, Gorgan, IR Iran AD - Faculty of Chemistry, Kharazmi University, Tehran, I.R. Iran AD - Department of Chemistry, Majlesi Branch, Islamic Azad University, Isfahan, I.R. Iran AD - Faculty of Chemistry, Islamic Azad University North Tehran Branch, Tehran, I.R. Iran Y1 - 2016 PY - 2016 VL - 3 IS - 2 SP - 265 EP - 278 KW - Chemiluminescence KW - Hydroxyzine KW - Cetirizine KW - Pharmaceuticals KW - Plasma DO - 10.22036/abcr.2016.39074 N2 - A chemiluminescence (CL) method has been developed for the determination of Hydroxyzine (Hyd) and its metabolite Cetirizine (Cet). The method is based on sensitizing effect of Cet and Hyd on the weak CL reaction between Ru(phen)32+ and acidic Ce(IV). A mechanism for the CL reaction has been proposed on the basis of UV-Vis, fluorescent and CL spectra. By using the recommended procedure, the calibration graphs were linear over 0.14 to 14.0 μg mL-1 (r2=0.9966) and 0.07 to 10.0 μg mL-1 (r2=0.9984) for Hyd and Cet, respectively. The limits of detections were 0.06 μg mL-1 and 0.03 μg mL-1 for Hyd and Cet, respectively. The percent of relative standard deviations (n=11) for 1.0 μg mL-1 of Hyd and 0.74 μg mL-1 of Cet were 4.5 and 2.3%, respectively. The broad time profile of Hyd and Cet allowed us to determine Hyd contents in plasma samples with minimum blank interferences from cysteine and ascorbic acid. The method has been satisfactorily used for the determination of Hyd and Cet in syrups, tablets and human plasma. UR - https://www.analchemres.org/article_39074.html L1 - https://www.analchemres.org/article_39074_2a09b6dbdc12b929fb49f9572b912b9e.pdf ER -