TY - JOUR ID - 9203 TI - Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics Methods JO - Analytical and Bioanalytical Chemistry Research JA - ABCR LA - en SN - AU - Madrakian, Tayyebeh AU - Madadi-Shad, Masoumeh AU - Soleimani, Mohammad AD - Bu- Ali Sina University, Hamedan, 6517838683, Iran AD - Department of Chemistry, Saveh Branch, Islamic Azad University, Saveh , Iran AD - Bu- Ali Sina University, Hamedan,6517838683, Iran Y1 - 2015 PY - 2015 VL - 2 IS - 1 SP - 42 EP - 51 KW - Mycophenolate mofetil KW - Mycophenolic acid KW - Simultaneous determination KW - Chemometric methods KW - Spectrophotometry DO - 10.22036/abcr.2015.9203 N2 - A spectrophotometric method for selective complexation reaction and simultaneous determination of mycophenolate mofetil (MPM), and mycophenolic acid (MPA) using three multivariate chemometric methods, i.e. partial least squares regression, principal component regression and principal component artificial neural networks, is proposed. The method is based on the complexation reaction of MPM and MPA with Fe(III) ion in the solution. A nonionic surfactant, Triton X-100, was used for dissolving the complexes and intensifying the signals. The linear determination ranges for the determination of MPA and MPM were 5.0-215.0 mg l-1, and 10.0-1000.0 mg l-1, respectively. The detection limit for MPA and MPM was obtained as 0.3 mg l-1 and 1.1 mg l-1, respectively. Satisfactory results were obtained by the combination of spectrophotometric method and chemometrics techniques. The method was successfully applied to the simultaneous determination of MPM and MPA in serum sample and the results were comparable with HPLC method. UR - https://www.analchemres.org/article_9203.html L1 - https://www.analchemres.org/article_9203_3bcac61326a10247b928d1afc40e5722.pdf ER -