Determination of the Volatile Components of Stachys Lavandulifolia with Periodic Mesoporous Organosilica as the Fiber Coating for Headspace Solid Phase Microextraction

Document Type: Research Paper


1 Department of Chemistry, Faculty of Science, University of Maragheh, Maragheh, Iran

2 Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran



A microwave-assisted headspace solid-phase microextraction technique (MA-HS-SPME) with a periodic mesoporous organosilica based on alkylimidazolium ionic liquid (PMO-IL) was created and utilized as a greatly porous fiber covering substance effectively in investigating the Stachys lavandulifolia’s essential oil composition. The specimen was exposed to microwave radiation and its volatile constituents were gathered via the fiber from the specimen headspace and straightly inserted into a GC-MS addition port for investigation. A simplex technique was utilized for optimizing 3 various factors influencing the extraction effectiveness. Under the enhanced circumstances (indeed the sample weight of 2 g, extraction time of 2.0 min and microwave power of 300 W), the PMO-IL nanoporous fiber could proficiently adsorb volatile components of Stachys lavandulifolia. In optimum conditions, the repeatability for one fiber (n = 3), expressed as relative standard deviation (R.S.D.%), was between 3.5% and 12.1% for the test compounds. The suggested technique, relative to hydrodistillation (HD) can equally be used to monitor all the sample components easily, but it will require less sample quantity and duration. A few experiments based on the simplex method proved it to be fast while an efficient method that can be used to optimize micro-extraction conditions.


[1]           D.M. Jiang, Q.H. Yang, H. Wang, G.R. Zhu, J. Yang, J. Catal. 239 (2006) 65.
[2]           S.L. Burkett, S.D. Sims, S. Mann, Chem. Commun. 11 (1996) 1367.
[3]           A. Mehdi, C. Reye, R. Corriu, Chem. Soc. Rev. 40 (2011) 563.
[4]           J.F. Liu, G.B. Jiang, Y.G. Chi, Y.Q. Cai, Q.X. Zhou, J.T. Hu, Anal. Chem. 75 (2003) 5870.
[5]           J.F. Liu, Y.G. Chi, G.B. Jiang, C. Tai, J.F. Peng, J.T. Hu, J. Chromatogr. A 1026 (2004) 143.
[6]           J.F. Peng, J.F. Liu, G.B. Jiang, C. Tai, J. Chromatogr. A 1072 (2005) 3.
[7]           D.W. Armstrong, L. He, Y.S. Liu, Anal. Chem. 71 (1999) 3873.
[8]           J.L. Anderson, D.W. Armstrong, Anal. Chem. 75 (2003) 4851.
[9]           J. Ding, T. Welton, D.W. Armstrong, Anal. Chem. 76 (2004) 6819.
[10]        S.J. Liu, F. Zhou, L. Zhao, X.H. Xiao, X. Liu, S.X. Jiang, Chem. Lett. 33 (2004) 496.
[11]        D.W. Armstrong, L.K. Zhang, L. He, M.L. Gross, Anal. Chem. 73 (2001) 3679.
[12]        M. Mank, B. Stahl, G. Boehm, Anal. Chem. 76 (2004) 2938.
[13]        M.Z. Moghaddam, E. Heinzle, A. Tholey, Rapid Commun. Mass Spectrom. 18 (2004) 141.
[14]        L. He, W. Zhang, L. Zhao, X. Liu, S.X. Jiang, J. Chromatogr. A 1007 (2003) 39.
[15]        J.F. Liu, N. Li, G.B. Jiang, J.M. Liu, J.A. Jonsson, M.J. Wen,  J. Chromatogr. A 1066 (2005) 27.
[16]        B. Karimi, D. Elhamifar, J.H. Clark, A.J. Hunt, Chem. Eur. J. 16 (2010) 8047.
[17]        M.M. Abolghasemi, B. Karimi, V. Yousefi, Anal. Chim. Act. 804 (2013) 280.
[18]        M.M. Abolghasemi, M. Piryaei, Chemija 23 (2012) 244.
[19]        A. Ghasemi Pirbalouti, M. Mohammadi, Asian Pac J Trop Biomed. 3 (2013) 123.
[20]        B. Karimi, D. Enders, Org. Lett. 8 (2006) 1237.
[21]        M.M. Abolghasemi, M. Piryaei, Chemija 23 (2012) 244.
[22]        B. Karimi, D. Elhamifar, J.H. Clark, A.J. Hunt, Chem. Eur. J. 16 (2010) 8047.
[23]        P. Hashemi, M.M. Abolghasemi, R. Ghiasvand, S. ahmadi, H. hassanvand, A. Yarahmadi, Chromatographia 69 (2009) 179.