Reverse Phase HPLC Method for the Simultaneous Determination of Cetirizine, Verapamil/Diltiazem and Amlodipine

Document Type : Research Paper

Authors

1 Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, University of Karachi, Karachi, 75270, Pakistan

2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi

3 Chemistry Department, Faculty of Applied Sciences, Umm Al-Qura University, Makkah, Saudi Arabia

Abstract

Determination of cetirizine, diltiazem, or verapamil and amlodipine in an active and in dosage formulations has been performed using a simple RP-HPLC method. Rosuvastatin is used in this novel RP-HPLC method as an internal standard to improve the selectivity of the method. At 230 nm, the separation was performed using a mobile phase consisting of methanol, acetonitrile, and water mixture in a ratio of 65:5:30, and pH at 2.8 allowed improved separation and faster times of analysis. ICH guidelines have pursued the validation of the method by assessing accuracy, precision (%RSD>2), and linearity (>0.999). The retention times of diltiazem, verapamil, amlodipine, cetirizine, and rosuvastatin was found to be 2.5, 3.2, 4.1, and 6 minutes, respectively. Method specificity was good as no interference of excipients of the tablets were observed in the analysis. The developed method could be used for routine quality control and in biological samples for the analysis of these drugs.

Keywords

References

[1]        E. Braunwald, N. Engl, J. Med. 307 (1982) 1618.
[2]        S. Kony, M. Zureik, C. Neukirch, B. Leynaert, D. Vervloet, F. Neukirch, Am. J. Resp. Crit. Care Med. 167 (2003) 538.
[3]        F. Hertz, M. Ranson, F.V. DeFeudis, Drug Develop. Res. 3 (2004) 331.
[4]        J.H. Stebbings, Am. J. Epidemiol. 97 (1973) 4.
[5]        M.A. Garcı́a, C. Soláns, J.J. Aramayona, L.J. Fraile, M.A. Bregante, J.R. Castillo, Talanta 47 (1998) 1245.
[6]        M. Çelebier, M.S. Kaynak, S. Altınöz, S. Şahin, Hacettepe University Journal of the Faculty of Pharmacy 28 (2008) 15.
[7]        D.N. Vora, A.A. Kadav, Indian Journal Of Pharmaceutical Sciences 70(2008) 542.
[8]        B.G. Chaudhari, N.M. Patel, P.B. Shah, Chem. Pharm. Bull. 55 (2007) 241.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
[9]        K.R. Naidu, U.N. Kale, M.S. Shingare, J. Pharm. Biomed. Anal. 39 (2005) 147.
[10]    A.Z. Mirza, M.S. Arayne, N. Sultana, Anal. Chem. Lett. 4 (2014) 302.
[11]    M.M. Bkhaitan, A.Z. Mirza, Bull. Korean Chem. Soc. 36 (2015) 2230.
[12]    M.S. Arayne, N. Sultana, A.Z. Mirza, J. Chromatogr. Sci. 49 (2011) 114.
[13]    N. Sultana, M.S. Arayne, H. Shamshad, J. Chem. Soc. Pakistan 30 (2008) 563.
[14]    N. Sultana, M.S. Arayne, H. Shamshad, Med. Chem. Res. 19 (2010) 462.
[15]    H. Shamshad, N. Sultana, M.S. Arayne, Anal. Chem. Lett. 5 (2015) 109.
[16]    N. Sher,  F.A. Siddiqui,  N. Hasan,  N. Shafi, A. Zubair, A.Z. Mirza, Anal. Methods 6 (2014) 2704.
[17]    ICH harmonized Tripartite Guideline, 2006, Q2(R1).
 
 
 
 
Analytical and Bioanalytical Chemistry Research
Volume 8, Issue 2
April 2021
Pages 139-145

Files

Share

How to cite

Statistics