Aptamer-based Electrochemical Detection of Tyrosinamide Using Metal-organic Frameworks/silver Nanoparticles Modified Glassy Carbon Electrode

Document Type : Research Paper

Authors

1 Department of Chemistry, Faculty of Sciences, Ilam University, Ilam, Iran

2 Department of Chemistry, Khorramabad Branch Islamic Azad University Khorramabad, Iran

Abstract

In this report, a new aptamer-based assay was presented reporting the electrochemical aptasensing for sensing tyrosinamide (Tyr-NH2). This strategy was relied on unbeatable conformational flexibility and specific recognition of aptamers. The tyrosinamide aptamer (Tyr-NH2-aptamer) was immobilized onto the metal-organic frameworks/silver nanoparticles modified glassy carbon electrode and hexacyanoferrate was selected as a probe to monitor interface variations during modification of the electrode and the aptamer conformational change generated by the Tyr-NH2 binding. Results showed that measurements by using electrochemical impedance spectroscopy had linear with the Tyr-NH2 concentrations in range of 0.01-0.25 nM and 0.25-1.15 nM. Detection limit of this system was found to be 2.3 pM. This method was also used to the Tyr-NH2 detection in serum samples successfully. Remarkable simplicity, ease of use and low-cost, make methodology as sensitive analytical system for sensing of the Tyr-NH2 that can be miniaturized. This strategy offers some promising advantages in reliable detection of the Tyr-NH2, which may be helpful in the routine analysis.

Keywords

References

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Analytical and Bioanalytical Chemistry Research
Volume 8, Issue 3
July 2021
Pages 275-283

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