Pharmaceutical and Bio-analytical Applications of Ion Mobility Spectrometry for Determination of Clopidogrel (Plavix)

Document Type : Research Paper


IAU, Yazd branch



Clopidogrel with trade name plavix, is used effectively to reduce the incidence of ischaemic strokes, heart attacks and claudication. In this study, an ion mobility spectrometry (IMS) method was proposed for the clopidogrel determination in analytical and bio-analytical fields. Under experimental conditions, the analytical validation parameters of the proposed method were calculated and reported. The calibration graph was linear in range 30.0–2000.0 µg mL-1 for clopidogrel with two orders of magnitude and a goodness of fit, R > 0.99. The coefficient of variation (precision, n=10) was found to be within 7.5–8.6%, and also the concentration levels of 3.0 and 10.0 µg mL-1 from analyte were obtained for the limits of detection and quantification, respectively. The developed IMS method was successfully applied to determine clopidogrel with accurate recovery range (90.0–103.7%) in different matrices. The validation studies showed that the applied IMS was a simple, rapid, sensitive and reliable method for the clopidogrel analysis in the pharmaceutical and biological samples.


[1]           K. Jose, P. Jayasehar, Int. J. Compr. Pharm. 1 (2013) 1.
[2] , The Internet Drug Index for prescription drugs, medication and pill identifier, Available from:
[3]           G. Patti, G. Micieli, C. Cimminiello, L. Bolognese, Cardiovasc. Ther. Volume 2020, 12 pages.
[4]           J.M. Pereillo, M. Maftouh, A. Andrieu, M.F. Uzabiaga, O. Fedeli, P. Savi, M. Pascal, J.M. Herbert, J.P. Maffrand, C. Picard, Drug Metab. Dispos. 30 (2002) 1288.
[5]           A. Wisniewski, K. Filipska, Int. J. Mol. Sci. 21 (2020) 6408.
[6]           P. Savi, J.L. Zachayus, N. Delesque-Touchard, C. Labouret, C. Hervé, M.F. Uzabiaga, J.M. Pereillo, J.M. Culouscou, F. Bono, P. Ferrara, J.M. Herbert, Proc. Natl. Acad. Sci. USA 103 (2006) 11069.
[7]           P.B. Chaudhari, P.D. Pawar, K.P. Narkhede, Int. J. Res. Ayurveda. Pharm. 1 (2010) 418.
[8]           D. Antic, S. Filipic, D. Agbaba, Acta Chromatogr. 18 (2007) 199.
[10]        A. Santhy, B. Saraswathyamma, P.Uma Sankar, R.Vidya, R. Rejithamol, Mater. Today-Proc. 5 (2018) 17812.
[11]        Y. Gomez, E. Adams, J. Hoogmartens, J. Pharm. Biomed. Anal. 34 (2004) 341.
[13]        K.M. Rao, K.R. Amperayani, K. Deepti, P.U. Devi, J. Indian Chem. Soc. 93 (2016) 1.
[14]        L. Gangyi, D. Chunxia, S. Weiwei, X. Lu, M. Zhang, Y. Gui, Q. Zhoua, C. Yu, Acta Pharm. Sinic. B 6 (2016) 55.
[15]        N.P. Gosavi, M.U. Bhajane, V.V. Patil. V.R. Patil, Res. J. Pharm. Biol. Chem. Sci. 3 (2012) 1065.
[16]        N.K. Sahoo, M. Sahu, P.S. Rao, J.N. Indira, N.S. Rani, G. Ghosh, J. Taibah Uni. Sci. 8 (2014) 331.
[17]        M.A. Al-Khayat, S. Haidar, H. Mando, Int. J. Pharm. Sci. Rev. Res. 14 (2012) 1.
[18]        S. Dermis, E. Aydogan, Pharmazi. 65 (2010) 175.
[19]        A.F. Khorshid, Arab. J. Chem. 12 (2019) 1740.
[20]        A.S. Fayed, S.A. Weshahy, M.A. Shehata, N.Y. Hassan, J. Pauwels, J. Hoogmartens, A. Van Schepdael, J. Pharma. Biomed. Anal. 49 (2009) 193.
[21]        M. Li, H. Ma , J. Gao, L. Zhang, X. Wang, D. Liu, J. Bian, Y. Jiang, J. Pharm. Biomed. Anal. 25 (2017) 203.
[22]        M. Tzschoppe, H. Haase, M. Höhnisch, D. Jaros, H. Rohm, Food Control 64 (2016) 17.
[23]        N. Ghotbadini-Bahraman, A. Sheibani, M.R. Shishehbore, Int. J. Ion Mobil. Spect. 20 (2017) 41.
[24]        Y. Valadbeigi, V. Ilbeigi, W. Mamozai, M. Soleimani, J. Pharm. Biomed. Anal. 197 (2021) 113980.
[25]        M. Li, W. Huang, H. Chen, D. Jiang, W. Wang, Y. Xiao, C. Chen, H. Li, Sensor Actuat. B-Chem. 330 (2021) 129365.
[26]        F. Zamani, B. Farajmand, M.R. Yaftian, Microchem. J. 159 (2020) 105540.
[27]        R.V. Nirogi, V.N. Kandikere, M. Shukla, K. Mudigonda, D.R. Ajjala, J. Chromatogr. B 848 (2007) 271.
[28]        Y. Ardakani, M.R. Rouini, J. Pharm. Biomed. 44 (2007) 1168.
[29]        M.J. Bogusz, R.D. Maier, K.D. Kruger, U. Kohls, J. Anal. Toxicol. 22 (1998) 549.