Document Type : Research Paper
Pharmaceutical Sciences Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Medical Biology Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Due to the inherent properties of electrochemical immunosensors, they are widely considered to detect various biomarkers. This study developed an electrochemical immunosensor via the excellent catalytic effect of nitrogen-doped graphene quantum dot supported by graphene nanoribbon (N-GQD/GNR) composite as a sensing platform. Nafion was used as a binder for binding of N-GQD/GNRon the electrode surface . The modified electrode with N-GQD/GNR composite was applied to develop the label-free electrochemical immunosensor for the measurements of carbohydrate antigen 15-3 (CA15-3) biomarker. The catalytic activity of N-GQD/GNR nanocomposite increases the peak currents of the proposed immunosensor due to the accelerated electron transfer between the sensing platform and probe. This immunosensor has a wide linear range of 0.5 to 150.0 U mL-1, with a detection limit of 0.1 U mL-1. Also, the proposed immunosensor has significant specificity, high sensitivity, and accuracy. The prepared electrochemical immunosensor was used to detect CA15-3 protein in blood samples.