Document Type : Research Paper
Authors
1
Department of Food Hygiene, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran
2
Department of Food Hygiene, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran. Health Promotion Research Center, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran
3
Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran. Engineering Faculty, Near East University, 99138 Nicosia, North Cyprus, Mersin 10, Turkey
Abstract
A facile and efficient stir bar sorptive extraction method was developed for the extraction of four antiparasitic drugs including eprinomectin, doramectin, ivermectin, and abamectin from cow milk samples. The extracted analytes were determined by high performance liquid chromatography-diode array detector. In this work, firstly, milk sample was deproteinized and the obtained clear solution was extracted by a stir bar coated with octadecylsilane (as the sorbent). After extraction, the adsorbed analytes onto the sorbent surface were eluted by a proper volume of 1-butyl-3-methylimidazolium tetrafluoroborate as ionic liquid. All of the effective parameters including sorbent type and amount, elution solvent type and volume, stirring time, deproteinization agent solution volume and concentration were investigated by design of expert using response surface methodology. Under optimal conditions, acceptable extraction recoveries (68 to 83%) and enrichment factors (72 to 88), low limits of determination (0.05 to 0.12 ng/mL) and quantification (0.17–0.41 ng/mL) were obtained. The method precision was evaluated by analyzing the spiked samples using the introduced method in a day and different days. The obtained relative standard deviations were in the ranges of 2.3-4.1 and 2.8-4.9%, for intra- and inter-days repeatability, respectively. At last, the presence of studied drugs in milk samples was followed and ivermectin was found in some samples. The literature review verified that the method was not performed previously and can be utilized as a routine method for analyzing antiparasitic drugs.
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