Electrocatalytic Determination of Isoniazid by a Glassy Carbon Electrode Modified with Poly (Eriochrome Black T)

Document Type: Research Paper

Authors

1 Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran

2 Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran

Abstract

In this work poly eriochrome black T (EBT) was electrochemically synthesized on the glassy carbon electrode as electrode modifier. On the modified electrode, voltammetric behavior of isoniazid (INH) was investigated. The poly (EBT)-modified glassy carbon electrode has excellent electrocatalytic ability for the electrooxidation of isoniazid. This fact was appeared as a reduced overpotential of INH oxidation in a wide operational pH range from 2 to 13. It has been found that the catalytic peak current depends on the concentration of INH and solution pH. The number of electrons involved in the rate determining step was found 1. The diffusion coefficient of isoniazid was also estimated using chronoamperometry technique. The experimental results showed that the mediated oxidation peak current of isoniazid is linearly dependent on the concentration of isoniazid in the ranges of 8.0 × 10-6 – 1.18 × 10-3 M and 2.90 × 10-5 M – 1.67× 10-3 M with differential pulse voltammetry (DPV) and amperometry methods, respectively. The detection limits (S/N = 3) were found to be 6.0 μM and 16.4 μM by DPV and amperometry methods, respectively. This developed method was applied to the determination of isoniazid in tablet samples with satisfactory results.

Keywords


[1] World Health Organization, Global Tuberculosis Report 2012, 2012, 3.

[2] USP DI®, Drug Information for the Health Care Professional, vol. I, 15th ed., (1995) 1627.

[3] M.R. Majidi, A. Jouyban, K. Asadpour-Zeynali, Genetic Algorithm Based Potential Selection in Simultaneous Voltammetric Determination of Isoniazid and Hydrazine by Using Partial Least Squares (PLS) and Artificial Neural Networks (ANNs), Electroanal. 17 (2005) 915–918.

[4] C.J. Shishoo and M.B. Devani, Nonaqueous titrimetric determination of isoniazid in presence of excess of sodium p-aminosalicylate in dosage forms, J. Pharm. Sci. 59 (1970) 92–93.

[5] K.K. Verma, S. Palod, The titrimetric determination of 4-pyridine carboxylic acid hydrazide (isoniazid) in drug formulations with thallium (III), Anal. Lett. 18 (1985) 11–19.

[6] A.H.N. Ahmed, S.M.E. Gizawy, H.I.E. Subbagh, Spectrophotometric determination of isoniazid using ethyl 8-quinolyloxyacetate, Anal. Lett. 25 (1992) 73–80.

[7] P. Nagaraja, K.C.S. Murthy, H.S. Yathirajan, Spectrophotometric determination of isoniazid with sodium 1,2-naphthoquinone-4-sulphotlate and cetyltrimethyl ammonium bromide, Talanta 43 (1996) 1075–1080.

[8] S.A. Benetton, E.R.M. Kedor-Hackmann, M. Santoro, V.M. Borges, Visible spectrophotometric and first-derivative UV spectrophotometric determination of rifampicin and isoniazid in pharmaceutical preparations, Talanta 47 (1998) 639–643.

[9] B.G. Gowda, M.B. Melwanki, J. Seetharamappa, K.C.S. Srinivasa Murthy, Spectrophotometric determination of isoniazid in pure pharmaceutical formulations, Anal. Sci. 18 (2002) 839-841.

[10] A. Safavi, M.A. Karimi, N.M.R. Hormozi, Sensitive indirect spectrophotometric determination of isoniazid, Spectrochim. Acta Part A 60 (2004) 765–769.

[11] Q.M. Li, Z.J. Yang, Spectrophotometric study of isoniazid by using 1,2-naphthoquinone-4-sulfonic acid sodium as the chemical derivative chromogenic reagent, J. Chin. Chem. Soc. 53 (2006) 383–389.

[12] H. Zhang, L. Wu, Q. Li, X. Du, Determination of isoniazid among pharmaceutical samples and the patients' saliva samples by using potassium ferricyanide as spectroscopic probe reagent, Anal. Chim. Acta 628 (2008) 67–72.

[13] E.F. Oga, Spectrophotometric determination of isoniazid in pure and pharmaceutical formulations using vanillin, Int. J. Pharm. Pharm. Sci. 2 (2010) 55–58.

[14] A. Safavi, M.A. Karimi, M.R.H. Nezhad, Flow injection determination of isoniazid using N-bromosuccinimide and N-chlorosuccinimide–luminol chemiluminescence system, J. Pharm. Biomed. Anal. 30 (2003) 1499–1506.

[15] Y. Xiong, H.J. Zhou, Z.J. Zhang, D.Y. He, C. He, Flow-injection chemiluminescence sensor for determination of isoniazid in urine sample based on molecularly imprinted polymer, Spectrochim. Acta Part A 66 (2007) 341–346.

[16] B. Haghighi, S. Bozorgzadeh, Flow injection chemiluminescence determination of isoniazid using luminol and silver nanoparticles, J. Microchem. 95 (2010) 192–197.

[17] P.C. Ioannou, A simple and rapid fluorimetric method for the microdetermination of isonicotinic acid hydrazide, Talanta 34 (1987) 857–860.

[18] R.A.S. Lapa, J. Lima, J.L.M. Santos, Fluorimetric determination of isoniazid by oxidation with cerium (IV) in a multicommutated flow system, Anal. Chim. Acta 419 (2000) 17–23.

[19] J.O. Svensson, A. Muchtar, and O. Ericsson. Ion-pair high-performance liquid chromatographic determination of isoniazid and acetylisoniazid in plasma and urine. Application for acetylator phenotyping, J. Chromatogr.341 (1985)193–97.

[20] H.I. Seifart,W.L. Gent, D.P. Parkin, P.P. van Jaarsveld, P.R. Donald, High-performance liquid chromatographic determination of isoniazid, acetylisoniazid and hydrazine in biological fluids, J. Chromatogr. B 674 (1995) 269–275.

[21] E. Calleri, E.D. Lorenzi, S. Furlanetto, Validation of a RP-LC method for the simultaneous determination of isoniazid, pyrazinamide and rifampicin in a pharmaceutical formulation, J. Pharma. Biomed. Anal. 29 (2002) 1089–1096.

[22] S. Guermouche, M.H. Guermouche, Solid-phase extraction and HPTLC determination of isoniazid and acetylisoniazid in serum. Comparison with HPLC, J. Chromatogr. Sci. 42 (2004) 250–253.

[23] M.Y. Khuhawar, L.A. Zardari, Capillary gas chromatographic determination of isoniazid in pharmaceutical preparations and blood by precolumn derivatization with trifluoroacetylacetone, J. Food Drug Anal. 14 (2006) 323–328.

[24] R. Milán-Segovia, G. Pérez-Flores, J.D. Torres-Tirado, X. Hermosillo-Ramírez, M. Vigna-Pérez, S. Romano-Moreno, Simultaneous HPLC determination of isoniazid and acetylisoniazid in plasma, Acta Chromatogr. 19 (2007) 110–118.

[25] P.F. Fang, H.L. Cai, H.D. Li, R.H. Zhu, Q.Y. Tan, W. Gao, P. Xu, Y.P. Liu, W.Y. Zhang, Y.C. Chen, F. Zhang, Simultaneous determination of isoniazid, rifampicin, levofloxacin in mouse tissues and plasma by high performance liquid chromatography– tandem mass spectrometry, J. Chromatogr. B 878 (2010) 2286–2291.

[26] P. Liu, Z. Fu, J. Jiang, L. Yuan and Z. Lin, Determination of isoniazid concentration in rabbit vertebrae by isotope tracing technique in conjunction with HPLC, Biomed. Chromatogr. 27 (2013) 1150–1156.

[27] T.Y. You, L. Niu, J.Y. Gui, S.J. Dong, E.K. Wang, Detection of hydrazine, methylhydrazine and isoniazid by capillary electrophoresis with a 4-pyridyl hydroquinone self-assembled microdisk platinum electrode, J. Pharm. Biomed. Anal. 19 (1999) 231–237.

[28] X. Zhang, Y. Xuan, A. Sun, Y. Lv, X. Hou, Simultaneous determination of isoniazid and p-aminosalicylic acid by capillary electrophoresis using chemiluminescence detection, Luminescence 24 (2009) 243–249.

[29] M.H. Shah, J.T. Stewart, Amperometric determination of isoniazid in a flowing stream at the glassy carbon electrode, Anal. Lett. 16 (1983) 913–923.

[30] M.A.A. Lomillo, O.D. Renedo, M.J.A. Martínez, Resolution of ternary mixtures of rifampicin, isoniazid and pyrazinamide by differential pulse polarography and partial least squares method, Anal. Chim. Acta 449 (2001) 167–177.

[31] M.M. Ghoneim, K.Y. El-Baradie, A. Tawfik, Electrochemical behavior of the antituberculosis drug isoniazid and its square-wave adsorptive stripping voltammetric estimation in bulk form, tablets and biological fluids at a mercury electrode, J. Pharm. Biomed. Anal. 33 (2003) 673–685.

[32] H.Y. Xia, X.Y. Hu, Determination of isoniazid using a gold electrode by differential pulse voltammetry, Anal. Lett. 38 (2005) 1405–1414.

[33] M.R. Majidi, A. Jouyban, K. Asadpour-Zeynali, Voltammetric behavior and determination of isoniazid in pharmaceuticals by using overoxidized polypyrrole glassy carbon modified electrode, J. Electroanal. Chem. 589 (2006) 32–37.

[34] G.J. Yang, C.X. Wang, R. Zhang, C.Y. Wang, Q.S. Qu, X.Y. Hu, Poly(amidosulfonic acid) modified glassy carbon electrode for determination of isoniazid in pharmaceuticals, Bioelectrochem. 73 (2008) 37–42.

[35] M.F. Bergamini, D.P. Santos, M.V.B. Zanoni, Determination of isoniazid in human urine using screen-printed carbon electrode modified with poly-L-histidine, Bioelectrochemistry 77 (2010) 133–138.

[36] K. Asadpour-Zeynali, P. Soheili-Azad, Simultaneous polarographic determination of isoniazid and rifampicin by differential pulse polarography method and support vector regression, Electrochim. Acta 55 (2010) 6570-6576.

[37] N.F. Atta, A. Galal, R.A. Ahmed, Voltammetric behavior and determination of isoniazid using PEDOT electrode in presence of surface active agents, Int. J. Electrochem. Sci. 6 (2011) 5097–5113.

[38] S. Shahrokhian and M. Amiri, Multi-walled carbon nanotube paste electrode for selective voltammetric detection of isoniazid, Microchim. Acta 157 (2007) 149–158.

[39] H. Yao, Y. Sun, X. Lin, Y. Tang, L. Ailin, Li. Guangwen, L. Wei and Z. Shaobo, Selective Determination of Epinephrine in the Presence of Ascorbic Acid and Uric Acid by Electrocatalytic Oxidation at Poly (Eriochrome Black T) Film-Modified Glassy Carbon Electrode, Anal. Sci. 23 (2007) 677–682.

[40] H. Yao, Y. Sun, X. Lin, Y. Tang, and L. Haung, Electrochemical Characterization of Poly (Erichrome Black T) Modified Glassy Carbon Electrode and its Application to Simultaneous Determination of Dopamine, Ascorbic Acid and Uric Acid, Electrochim. Acta 52 (2007) 6165–6171.

[41] U. Chandra, B.E.K. Swamy, O. Gilbert, S. Reddy, B.Sh. Sherigara, Determination of Dopamine in Presence of Uric Acid at Poly (Eriochrome Black T) Film Modified Graphite Pencil Electrode, American J. Anal. Chem. 2 (2011) 262–269.

[42] S. M. Golabi and H. R. Zare, Electroanalysis 11 (1999) 1293–1300.

[43] A.J. Bard, L.R. Faulkner, Electrochemical Methods, Fundamentals and Applications, Wiley, New York, 1980, pp 103-104.

[44] U. Pratap Azad and Ve. Ganesan, Efficient electrocatalytic oxidation and selective determination of isoniazid by Fe(tmphen)32+-exchanged Nafion®-modified electrode, J. Solid State Electrochem. 16 (2012) 2907–2911.