Chemometrics-enhanced Kinetic Spectrophotometric Method for Simultaneous Determination of Ag+, Cu2+ and Ni2+ Ions in Some Medicinal Plants by Dimethyl 2,2’-(ethan-1,2-diylbis1)bis(cyclopent-1-ene-1-carbodithioate)

Document Type: Research Paper


1 Department of chemistry, faculty of science, Persian Gulf university,Bushehr, iran

2 Department of Chemistry, Faculty of Sciences, Persian Gulf University, Bushehr 75169, Iran

3 aDepartment of Chemistry, Faculty of Sciences, Persian Gulf University, Bushehr, 75169, Iran

4 Department of Biotechnology, Persian Gulf Institute, Persian Gulf University, Bushehr, 75169, Iran

5 Department of Chemistry, Faculty of Sciences, Persian Gulf University, Bushehr, 75169, Iran


The metal ions constituents in plants reliable for determination of their medicinal, nutritional and toxicity properties. A novel, simple and accurate spectrophotometric method was developed for the simultaneous determination of Ag+, Cu2+ and Ni2+ ions in different medicinal plants without prior separation steps. This method was based on the different kinetic characteristics between the reactions of analytes with newly synthesized Schiff base as dimethyl 2,2’,-(ethan-1,2-diylbis1)bis(cyclopent-1-ene-1-carbodithioate(DEBC). All experimental conditions include, DEBC concentration, effects of pH and temperature was optimized. Also, orthogonal array design was applied for the construction of concentration. The differential kinetic spectra were monitored and recorded at 397 nm. The reaction orders were estimated with respect to complex mixture of analytes with DEBC. Limit of detections values were 0.013, 0.078 and 0.003 mg L-1 and limit of quantifications values were 0.043, 0.250 and 0.011 for, Cu2+, Ni2+ and Ag+, respectively. The recorded data were processed by principal component analysis- back propagation neural networks (PCBPNNs). A set of synthetic mixtures of metal ions was evaluated and the obtained results by PCBPNNs were discussed. The simultaneous analysis was also performed without compressed data and was compared with those obtained by PCBPNNs. The proposed method was successfully applied to the simultaneous determination of metal ions in medicinal plants samples. The results of proposed method were compared by those obtained on the same samples by Graphite furnace atomic absorption spectrometry.


[1]       M. Rahimi, R. Farhadi, M. Salehi Balashahri, Intl. J. Agron Plant Prod. 3 (2012) 154.
[2]       S. Basgel, S.B. Erdemoğlu, Sci. Total Environ. 359 (2006) 82.
[3]       G.L. Eichhorn, Mech. Ageing DeV. 9 (1979) 291.
[4]       F.V. Assche, H. Clijsters, Plant Cell and Environ. 13 (1990) 195.
[5]       A.D. Kaur, V. Ravichandran, P.K. Jain, J. Pharm.  Biomed.  Anal. 46 (2008) 391.
[6]       Ł. Cieśla, A. Bogucka-Kocka, M. Hajnos, J. Chromatogr.  A 1207 (2008) 160.
[7]       Y. Jiang, B. David, P. Tu, Anal. Chim. Acta 657 (2010) 9.
[8]       Y Jin, T. Liang, Q. Fu, J. Chromatogr. A 1216 (2009) 2136.
[9]       I. Bombarda, N.J.P.D. Dupuy, Anal. Chim.  Acta 613 (2008) 31.
[10]    H. Zhu, Wang, Y.H. Liang, G. Chen, P. Zhao, J. Tao, Talanta 81 (2010) 129.
[11]    N. Djabou, M.J. Battesti, H. Allali, L. Phytochemistry 72 (2011) 1390.
[12]    I.A. Suther, D. Fisher, J Chromatogr. A 1216 (2009) 740.
[13]    N.B. Kormušoska, K. Čundeva, T. Stafilov, J. Environ. Sci. Heal A 44 (2009) 1274.
[14]    J.T. Kinard, J. Environ. Sci. Heal A 12 (1977) 531.
[15]    B.K.P. Huang, L.Q. Huang, L.P. Qin, Z.X. Zhao, H.C. Zheng, L. J. Chin.  Med.  Mater. 31 (2008) 1494.
 [16]    H.S. Chen, R.B. Bhavik, K.G. Prem, Anal. Chim. Acta 602 (2007) 1.
[17]    F. Gong, Y.Z. Liang, Y.S. Fung, J. Chromatogr. A 1029 (2004) 173.
[18]    H.L. Zhai, F.D. Hu, X.Y. Huang, J.H. Chen, Anal. Chim. Acta 657 (2010) 131.
[19]    I.A. Sutherl, DerekFisher, J. Chromatogr. A 1216 (2009) 740
[20]    R.T. Tian, P.S. Xie, H.P. Liu, J. Chromatogr. A 1216 (2009) 2150.
[21]    F.C. Sanchez, J. Toft, B. Vanden Bogaert, Anal. Chem. 68 (1996) 79.
[22]    C.J. Xu, Y.Z. Liang, F.T. Chau, J. Chromatogr. A 1134 (2006) 253.
[23]    Y.X. Zeng, C.X. Zhao, Y.Z. Liang, Anal. Chim. Acta 595 (2007) 328.
[24]    O.M. Kvalheim, Y.Z. Liang, Anal. Chem. 64 (1992) 936.
[25]    A. Afkhami, M. Abbasi Tarighat, Talanta 78 (2009) 424.
[26]    A. Afkhami, M. Abbasi Tarighat, H. Khanmohammadi, Talanta 77 (2009) 995.
[27]    J. Tisato, F. Refosco, F. Bandoli, Coord. Chem. Rev, 135 (1994) 325–397.
[28]    M. Asadi, Kh. Mohammadi, Sh. Esmaielzadeh, B. Etemadi, H. Fun, J. Inorg. Chim. Acta 362 (2009) 4913.
[29]    Kh. Mohammadi, M. Zahedi, Global J. Inorg. Chem. 3 (2012) 1.
[30]    W.G. Lan, M.K. Wong, N. Chen, Y.M. Sin, Analyst 119 (1994) 1669.
[31]    M. Abbasi Tarighat, M.R. Mohammadizadeh, Gh. Abdi, J. Agri. Food Chem. 61 (2103) 2632.
[32]    P.S. Hernandez, A.R. Hernandez, M.T. Ramiraz Silva, Spectrochim. Acta A 59 (2003) 2667.
[33]    I. Narin, M. Soylak, Anal. Chim. Acta 493 (2003) 205.
[34]    M. Ghaedi, A. Shokrollahi, F. Ahmadi, H.R. Rajabi, M. Soylak, J. Hazard. Mater. 150 (2008) 533.