Effects of Solvent, pH and Ionic Strength on the Fluorescence Features of Aflatoxin B1, B2, G1 and G2

Document Type: Research Paper

Authors

1 Department of Chemistry, University of Mazandaran, Babolsar, Iran.

2 Faculty of Chemistry, University of Mazandaran, Babolsar 47416-95447, Iran

3 Department of Chemistry, Bu-Ali Sina University, Hamedan, Iran.

4 Department of Chemistry, University of Mazandaran, Babolsar, Iran

Abstract

The effects of environmental parameters, including solvent, pH and ionic strength on the fluorescence features of aflatoxin B1, B2, G1 and G2 were investigated. Fluorescence spectral characteristics such as fluorescence emission intensity, stokes shift and maximum emission wavelength were markedly affected by solvent type. The emission fluorescence peak of aflatoxins has been assessed between 370 and 500 nm under a 360 nm excitation source. The maximum fluorescence intensities were obtained with ethanol and acetonitrile for all aflatoxins studied. The maximum displacement on emission wavelengths for aflatoxin B1, B2, G1 and G2 were obtained in methanol, dichloromethane, chloroform and acetonitrile, respectively. The study of the acidity showed that maximum signal intensity was obtained in relatively low acidity values. Also the study of ionic strength revolved that the maximum signals were obtained in 0.1 M NaCl. Also, this study shows that there is no need for another fluorophore for the study of investigated aflatoxins.

Keywords


[1]       B. Huanga, Z. Hanb, Z. Caia, Y. Wub, Y. Ren, Anal. Chim. Acta 62 (2010) 662.

[2]       International Agency for Research on Cancer (IARC). Monographs 6 on the Evaluation of Carcinogenic Risks to Humans. Lyon: IARC, 1993.

[3]       B. Guo, Z.Y. Chen, R. Dewey Lee, B.T. Scully, J. Integr. Plant Biol. 50 (2008) 1281.

[4]       J.W. Bennett, M. Klich, Clin. Microbiol. Rev. 16 (2003) 497.

[5]       H. Gourama, L.B. Bulleman, J. Food Prot. 58 (1995) 1395.

[6]       J.D. Miller, Food Addit. Contam. 25 (2008) 219.

[7]       S. Nawaz, R.D. Coker, S.J. Haswell, J. Planar Chromatogr. 8 (1995) 4.

[8]       Z. Fu, X. Huang, S. Min, J. Chromatogr. A 1209 (2008) 271.

[9]       N.W. Turner, S. Subrahmanyam, S. Piletsky, Anal. Chim. Acta 632 (2009) 168.

[10]    A.R. Pen, M.C. Alcaraz, L. Arce, A. Rios, M. Valcarcel, J. Chromatogr. A 967 (2002) 303.

[11]    H.K. Abbas, R.M. Zablotowicz, M.A. Weaver, B.W. Horn, W. Xie, W.T. Shier, J. Microbiol. 50 (2004) 193.

[12]    W.S. Khayoon, B. Saad, C.B. Yan, N.H. Hashi, A.S.M. Ali, M.I. Salleh, B. Salleh, Food Chem. 118 (2010) 882.

 

 

 

[13]    S.M. Herzallah, Food Chem. 114 (2009) 1141.

[14]    C.A.F. Oliveira, N.B. Gonc, R.E. Rosim, A.M. Fernandes, Int. J. Mol. Sci. 10 (2009) 174.

[15]    V.S. Sololev, J.W. Dorner, J. AOAC Int. 85 (2002) 642.

[16]    A. Korde, U. Pandey, S. Banerjee, H.D. Sarma, S. Hajare, M. Venkatesh, A.K. Sharma, M.R.A. Pillai, J. Agric. Food Chem. 51 (2003) 843.

[17]    C.M. Maragos, V.S. Thompson, Nat. Toxins 7 (1999) 371.

[18]    A.Y. Kolosova, W. Shim, Z. Yang, S.A. Eremin, D. Chung, Anal. Bioanal. Chem. 384 (2006) 286.

[19]    B. Sarimehmetoglu, O. Kuplulu, T. Haluk Celik, Food Control 15 (2004) 45.

[20]    S. Sforza, C. Dall’Asta, R. Marchelli, Mass Spectrom. Rev. 25 (2005) 54.

[21]    C. Cavaliere, P. Foglia, C. Guarino, M. Nazzari, R. Samperi, A. Lagana, Anal. Chim. Acta 141 (2007) 8.

[22]    A. Tarín, M.G. Rosell, X. Guardino, J. Chromatogr. A 1047 (2004) 235.

[23]    Y. Wang, T. Chai, G. Lu, C. Quan, H. Duan, M. Yao, Environ. Res. 107 (2008) 139.

[24]    D. Chan, S.J. MacDonald, V, Boughtflower, P. Brereton, J. Chromatogr. A 1059 (2004) 13.

[25]    J. Blesa, J.M. Soriano, J.C. Moltó, R. Marín, J. Mañes, J. Chromatogr. A 1011 (2003) 49.

[26]    I.K. Cigić, H. Prosen, Int. J. Mol. Sci. 10 (2009) 62.

[27]    M. Mably, M. Mankotia, P. Cavlovic, J. Tam, L. Wong, P. Pantazopoulos, P. Calway, P.M. Scott, Food Addit. Contam. 22 (2005) 1252.

[28]    J. Hashemi, G.A. Kram, N. Alizadeh, Talanta 75 (2008) 1075.

[29]    S.N. Shtykov, J. Anal. Chem. 57 (2002) 859.

[30]    I.Y. Goryacheva, T.Y. Rusanova, K.E. Pankin, J. Anal. Chem. 63 (2008) 751.

[31]    N.H.S. Ammida, L. Micheli, G. Palleschi, Anal. Chim. Acta 520 (2004) 159.

[32]    J.H.O. Owino, O.A. Arotiba, N. Hendricks, E.A. Songa, N. Jahed, T.T. Waryo, R.F. Ngece, P.G.L. Baker, E.I. Iwuoha, Sensors 8 (2008) 8262.

[33]    Y. Tan, X. Chu, G.L. Shen, R.Q. Yu, Anal. Biochem. 387 (2009) 82.