Electrochemical Detection of Celestine Blue Based on Screen Printed Carbon Electrode Modified with Molecular Imprinted Polymer and NiO Nanoparticles

Document Type : Research Paper


1 Department of Chemistry, Faculty of Science, Ilam University, Ilam, Iran

2 Department of Chemistry, Khorramabad Branch Islamic Azad University Khorramabad, Iran


A simple and rapid electrochemical method was developed for the detection of trace amount of Celestine blue (CB) at the surface of modified screen-printed carbon electrode (SPCE) with nickel oxide (NiO) nanoparticles and molecular imprinted polymer (MIP). Various types of electrochemical methods; containing, differential pulse voltammetry (DPV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in order to probe the characteristics of the sensor toward CB was employed. The selectivity, real sample analysis (efficiency), the linear concentration range, and the detection limit of CB at the MIP/NiO-NPs/SPCE were evaluated by DPV method. FT-IR spectroscopy was applied in order to characterize the synthesized CB-MIP and non-molecular imprinted polymers (NIP). Scanning electron microscopy (SEM) and BET (Brunauer-Emmett-Teller) techniques were used for surface morphology and porosity properties of CB-MIP and NIP. A linear range was presented by the sensor for CB concentration between 2-150 µM with detection limit (S/N=3) of 0.35 µM. The sensor is able to selectively detect the CB molecules over the potential interferences. The sensor was used to determine CB in the real sample with acceptable results.


[1]       A. Afkhami, H. Ghaedi, T. Madrakian, M. Ahmadi, H. Mahmood-Kashani, Biosens. Bioelectron. 44 (20130) 34.
[2]       J. Cheng,Y. Li,, J. Zhong, Z. La, G. Wang, M. Sun,Y. Jianga, P. Zou, X. Wang, Q. Zhao, Y. Wanga, H. Rao, Chem. Eng. J. 398 (2020) 125664.
[3]       A.E. Jaouhari, L. Yan, J. Zhu, D. Zhao, M.Z.H. Khan, X. Liu, Electrochim. Acta 1106 (2020) 103.
[4]       L. Ge, Sh. Wang, J. Yu, N. Li, Sh. Ge, M. Yan, Adv. Funct. Mater. 23 (2013) 3115.
[5]       R. Schirhagl, Anal. Chem. 86 (2014) 250.
[6]       N. Wang, S.J. Xiao, C.W. Su, Colloid Polym. Sci. 294 (2016) 1305.
[7]       W. Liana, J. Liang, L. Shen, Y. Jin, H. Liu, Biosens. Bioelectron. 100 (2018) 326.
[8]       A. Aghaei, M.R. Milani Hosseini, M. Najafi, Electrochim. Acta 55 (2010) 1503.
[9]       X.  Kan,  H.  Zhou,  C.  Li,  A. Zhu, Z. Xing, Z.  Zhao,
Electrochim. Acta 63 (2012) 69.
[10]    R. Pernites, R. Ponnapati, M.J. Felipe, R. Advincula, Biosens. Bioelectron. 26 (2011) 2766.
[11]    X. Kan, Z. Xing, A. Zhu, Z. Zhao, G. Xu, C. Li, H. Zhou, Sens. Actuators B: Chem. 168 (2012) 395.
[12]    W. Lian, J. Huang, J. Yu, X. Zhang, Q. Lin, X. He, X. Xing, S. Liu, Food Control 26 (2012) 620.
[13]    L. Özcan, M. Şahin, Y. Şahin, Sens. 8 (2008) 5792.
[14]    R. Kant, Nat. Sci. 4 (2012) 22.
[15]    A.V. Sokolov, V.A. Kostevich, S.O. Kozlov, I.S. Donskyi, I.I. Vlasova, A.O. Rudenko, E.T. Zakharova1, V.B. Vasilyev, O.M. Panasenko, Free Radic. Res. 49 (2015) 777.
[16]    Y. Liu, M.S. Ata, K. Shi, G.-Z. Zhu, G.A. Botton, I. Zhitomirsky, RSC Adv. 4 (2014) 29652.
[17]    R.L. McCreery, Chem. Rev. 108 (2008) 2646.
[18]    A.T. Lawal, Talanta 131 (2015) 424.
[19]    F. Taghizadeh, O. P. J. 6 (2016) 164.
[20]    V.S. Reddy Channu, R.  Holze, B. Rambabu, Colloids and Surfaces A: Physicochem. Eng. Aspects 414 (2012) 204.
[21]    H. Ebrahimzadeh, K. Molaei, A.A. Asgharinezhad, N. Shekari, Z. Dehghani, Anal. Chim. Acta 767 (2013) 155.
[22]    M. Arabi, M. Ghaedi, A. Ostovan, J. Tashkhourian, H. Asadallahzadeh, Ultrason. Sonochem 33 (2016) 67.
[23]    M. Roushani, A. Nezhadali, Z. Jalilian, A. Azadbakht, Mater. Sci Eng.: C 71 (2017) 1106.
[24]    A.J. Bard, L.R. Faulkner, Electrochemical Methods Fundamentals and Applications, Wiely, New York, 2001.
[25]    M. Roushani, E. Karami, Electroanalysis 26 (2014) 1761.