Development of a LC-MS/MS Method for the Determination of Narcotic Drugs and Psychotropic Substances in Human Hair

Document Type : Research Paper

Authors

1 Department of Medical Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey. Medical Biochemistry Verification Laboratory, Hacettepe University Hospitals, Ankara, Turkey

2 Medical Biochemistry Verification Laboratory, Hacettepe University Hospitals, Ankara, Turkey

3 Department of Basic Sciences, Faculty of Dentistry,Burdur Mehmet Akif Ersoy University, Burdur, Turkey

Abstract

Narcotic drug use is an important problem in our country and all over the world. Hair is a valuable specimen for monitoring these long-term drug uses. For this purpose, a sensitive method was created and validated for the identification and quantification of benzoylecgonine (, morphine, codeine, 6-monoacetylmorphine, heroin, tetrahydrocannabinol, amphetamine, 3,4-methylenedioxyamphetamine, buprenorphine, methamphetamine, 3,4-methylenedioxy-N-methylamphetamine, and cocaine in human hair by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The sample preparation step includes washing, standard addition, liquid-phase extraction with methanol, and solid-phase extraction steps. Analysis for substances identified from a pretreated hair took a maximum of 3.64 minutes. In the method, the LOD values of each substance ranged from 0.11-0.87 ng/mg, and the linearity was quite good (r2>0.99). The concentration ranges for quantification were 0.50 and 8.00ng/mg for all substances. In addition, the intraday and interday accuracy and precision values of this method were acceptable (˂12.81%) and the recovery was found to be between 93.72%-104.78% at different concentrations. The results of this study showed that the developed very fast and reliable LC-MS/MS method is suitable for use as a validated method in the analysis of illicit drugs simultaneously in hair.

Keywords