Assessment and Selective Extraction Megestrol Drug by Molecularly Imprinted Polymers Method in Human Fluid Samples Using Liquid Chromatography

Document Type : Research Paper


1 Department of Chemistry, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran

2 Department of Chemistry, Dashtestan Branch, Islamic Azad University, Dashtestan, Iran


In this study, a molecularly imprinted polymers-solid phase extraction-liquid chromatography method to isolate megestrol drug in human fluid samples. The molecularly imprinted polymers using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the crosslinking monomer, and 2, 2-Azoisobutyronitrile as an initiator for polymer preparation. To evaluate the applicability of the molecularly imprinted polymers was used as a selective sorbent, general parameters: pH, the amount of solvents, washing solution, and eluent, and time, were optimized following a step-by-step approach. Under the optimum conditions, the detection limit linear range was obtained (0.02 to 2.0 µgL-1). The relative standard deviation of (± 2.0 %), and the detection limit of the method (0.02 µg/L) were obtained. The recoveries up to approximately 97.0 % from spiked human fluid samples could be obtained. The maximum adsorption capacity for the determination of megestrol drugs was 6.8 mgg-1. The proposed molecularly imprinted polymers-solid phase extraction-liquid chromatography method could be applied to the direct determination of megestrol drugs in human fluid samples.