Calcium Hexacyanoferrate(II) as a Precipitating Reagent for the Determination of Mebeverine Using Multiple Flow Cells with Summed (S/N) Responses in NAG-4SX3-3D Instrument

Document Type : Research Paper


1 Department of Forensic Science, College of Science, Al-Karkh University of Science, Baghdad, Iraq

2 University of Baghdad, College of Science, Department of Chemistry, Baghdad, Al-Jadriya, Iraq

3 Department of chemistry, College of basic education, University of Mustansiryah, Iraq


A novel and highly sensitive flow injection method was developed for the determination of Mebeverine. The method is based on the formation of an ion pair compound between Mebeverine hydrochloride and calcium hexacyanoferrate in an ammonium chloride medium, resulting in the formation of a whitish-yellow precipitate complex. The analysis was performed using a homemade NAG-4SX3-3D triple flow cell CFIA Analyzer. The experimental parameters were studied to optimize both the chemical and physical aspects of the method. The linear working range was obtained to be 0.01-40 mmol L-1, with an impressive correlation coefficient (r) of 0.9907. The limit of detection (LOD) was determined to be 0.349 µg/150 µL, and the repeatability of the method (RSD%) was demonstrated to be less than 1.5%. To further evaluate the performance of the newly proposed method, a comparison was conducted using the standard addition method in conjunction with a classical method. Overall, the developed flow injection method offers simplicity, sensitivity, and reliable analytical performance for the determination of Mebeverine, making it a promising alternative to the classical method.