Chemical Analysis of Volatile Constituents of Pulicaria Mauritanica Isolated by Hydrodistillation and Headspace Solid-phase Micro-extraction Techniques. Antimicrobial Activity of its Essential Oil

Document Type : Research Paper



2 Université de Corse


The objective of this study is to report the comparative chemical analysis of essential oil (EO) obtained by hydrodistillation (HD) and volatile fraction (VF) detected by headspace solid phase micro-extraction (HS–SPME) isolated from Pulicaria mauritanica using Gas Chromatography–Retention Indices (GC-RI) and GC–Mass Spectrometry (GC-MS) and to evaluate in vitro the antimicrobial activity of P. mauritanica EO against three bacterial strains referenced by the ATCC (American Type Culture Collection): Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 27853) and a one yeast Candida albicans using the paper disc diffusion and minimum inhibitory concentration (MIC) assays. The chromatographic profile indicates that quantitative and semi qualitative differences between the chemical compositions of both analysed samples were observed. The antimicrobial activity tested by two methods indicates that this EO was more effective against three strains tested excepted P. aeruginosa which was most resistant. Indeed, the results of the report MBC (Minimum Bactericidal Concentration)/MIC show that the EO has a bactericidal effect on S. aureus and a bacteriostatic and fungistatic effect on E. coli and C. albicans, respectively. P. mauritanica EO seems be a promising source of natural products with potential antimicrobial activity.


[1]       J. Avorn, J.F. Barrett, P.G. Davey, S.A. McEwen, T.F. O’Brien,  S.B.  Levy,  W.H.  Organization, A.  for  the
P.U. of Antibiotics, Antibiotic Resistance: Synthesis of Recommendations by Expert Policy Groups, 2001.
[2]       K.S. N’Dédianhoua, K. Majdouli, K. Moutaouakil, F. El-Hilali, Y. Khabbal, A. Bentayeb, T. Zaïr, Int. J. Innov. Sci. Res. 9 (2014) 464.
[3]       W. Boyle, Americ. Perf. Essen. Oil Rev. 66 (1955) 25.
[4]       S. Burt, Int. J. Food Microbiol. 94 (2004) 223.
[5]       M. Lahlou, Phytoth. Res. 18 (2004) 435.
[6]       C.F. Carson, B.J. Mee, T.V. Riley, Antimicrob. Agents Chemoth. 46 (2002) 1914.
[7]       A. Poormohammadi, A. Bahramy, B. Shekher, Anal. Bioanal. Chem. Res. 6 (2019) 253.
[8]       L. Emberger, M. Chadefaud, Traité de botanique systématique: Les Végétaux Vasculaires, Par L. Emberger, Masson et Cie, 1960.
[9]       M. Grieve, A Modern Herbal. Penguin Books: Harmondsworth, UK, 1980 32.
[10]    A.N. Awadh, W.D. Julich, C. Kusnick, U. Lindequist, J. Ethnopharmacol. 74 (2001) 173.
[11]    M. Mahfouz, A. Ghazal, M. El-Dakhakhny, M.T. Ghoneim, J. Drug Res. 5 (1973) 151.
[12]    M. Znini, G. Cristofari, L. Majidi, J. Paolini, J.M. Desjobert, J. Costa, Food Sci. Technol. 54 (2013) 564.
[13]    D. Joulain, W.A. König, The Atlas of Spectral Data of Sesquiterpene Hydrocarbons, EB-Verlag, 1998.
[14]    D.H. Hochmuth, W.A. König, D. Joulain, Terpenoids and Related Constituents of Essential Oils. Library of Mass Finder 2.1, University of Hamburg, 2001.
[15]    Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard-ninth Edition. Pennsylvania, USA: CLSI, 2006.
[16]    A.J. Hayes, B. Markovic, Food Chem. Toxicol. 40 (2002) 535.
[17]    I. Wiegand, K. Hilpert, R. E Hancock, Nat. Protocol. 3 (2008) 163.
[18]    L. Scorzoni, T. Benaducci, A.M.F. Almeida, D.H.S. Silva, V. da S. Bolzani, M.J.S.M. Gianinni, Braz. J. Microbiol. 38 (2007) 391.
[19]    D. Gatsing, V. Tchakoute, D. Ngamga, J.-R. Kuiate, J.D.D. Tamokou, B.F. Nji-Nkah, F.M. Tchouanguep, S.P. Fodouop, Iran. J. Med. Sci. 34 (2015) 126.
[20]     M. Bammou, E.T. Bouhlali, K. Sellam, M. Ramchoun, A. McDonald, Y. Ouzidan, J. Ibijbijen,  L.
El Rhaffari, L. Nassiri, Amer. J. Essen. Oils Nat. Prod. 7 (2019) 07.
[21]    A. Hamdouch, A. Asdadi, F. Achemchem, B. Chebli, M. El Hadek, L.M. Idrissi Hassani, J. Mate. Envir. Sci. 9 (2018) 1762.
[22]    M. Gherib, B. Chahrazed, I.A. El-Haci, T.M. Chaouche, F.A. Bekkara, Int. J. Pharma. Sci. Res. 7 (2016) 76.
[23]    M. Gherib, C. Bekhechi, F.A. Bekkara, A. Bighelli, J. Casanova, F. Tomi, Nat. Prod. Commun. 11 (2016) 109.
[24]    T. Xu, M. Gherib, C. Bekhechi, F. Atik-Bekkara, H. Casabianca, F. Tomi, J. Casanova, A. Bighelli, Flavour Fragr. J. 30 (2015) 83.
[25]    M. Znini, L. Majidi, J.M. Desjobert, J. Paolini, J. Costa, Acta Chromatog. 26 (2014) 495.
[26]    M. Liang, M. Qi, C. Zhang, S. Zhou, R. Fu, J. Huang, Anal. Chim. Acta 531 (2005) 97.
[27]    J. Palá-Paúl, J.J. Brophy, R.J. Goldsack, B. Fontaniella, Biochem. System. Ecol. 32 (2004) 55.
[28]    A. Koedam, In Capillary Gas Chromatography in Essential Oil Analysis; Sandra P, Bicchi C, eds. Huethig, Verlag, New York, 1987.
[29]    J. Paolini, E. Nasica, J.-M. Desjobert, A. Muselli, A.-F. Bernardini, J. Costa, Phytochem. Anal. 19 (2008) 266.
[30]    N. Djabou, V. Lorenzi, E. Guinoiseau, S. Andreani, M.-C. Giuliani, J.-M. Desjobert, J.-M. Bolla, J. Costa, L. Berti, A. Luciani, Food Control 30 (2013) 354.
[31]    Clinical and Laboratory Standards Institute-Performance standards for antimicrobial Disk and Dilution Susceptibility Tests Methods for Antimicrobial Susceptibility Testing for Bacteria Isolated from Animals-Approved Standard-Third edition-CLSI Document M11-A7-Clinical and Laboratory Standards Institute, Wayne, PA, USA, 2007.
[32]    H.J.D. Domans, S.G. Deans, J. Appl. Microbiol. 88 (2000) 308.
[33]    A. Nasser, A. Awadh, F.S. Farukh, M. Alhaj, M.H. Gabrielle, A. Porzel, N. Arnold, W.N. Setzer, J. Schmidt, L. Wessjohann, Nat. Prod. Commun. 7 (2012) 257.
[34]    M. Al-Fatimi, N.A. Awadh, M. Wurster, S.S. Al-Sokari, Ul. Lindequist, W.N. Setzer, World J. Pharma. Res. 4 (2015) 1.
[36]    L. Panizzi, G. Flamini, P.L. Cioni, I. Morelli, J. Ethnopharmacol. 39 (1993) 167.
[37]    L. Kerbouche, M. Hazzit, A. Baaliouamer, J. Biolo Active Prod. Nat. 3 (2013) 266.
[38]    K. Oosterhaven, B. Poolman, E.J. Smid, Ind. Crops & Prod. 1 (1995) 23.
[39]    A. Zambonelli, A.Z. d’Aulerio, A. Bianchi, A. Albasini, J. Phytopathol. 144 (1996) 491.
[40]    A. Ultee, M.H.J. Bennik, R. Moezelaar, Appl. Envir. Microbiol. 68 (2002) 1561.