Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics Methods

Document Type: Research Paper


1 Bu- Ali Sina University, Hamedan, 6517838683, Iran

2 Department of Chemistry, Saveh Branch, Islamic Azad University, Saveh , Iran

3 Bu- Ali Sina University, Hamedan,6517838683, Iran


A spectrophotometric method for selective complexation reaction and simultaneous determination of mycophenolate mofetil (MPM), and mycophenolic acid (MPA) using three multivariate chemometric methods, i.e. partial least squares regression, principal component regression and principal component artificial neural networks, is proposed. The method is based on the complexation reaction of MPM and MPA with Fe(III) ion in the solution. A nonionic surfactant, Triton X-100, was used for dissolving the complexes and intensifying the signals. The linear determination ranges for the determination of MPA and MPM were 5.0-215.0 mg l-1, and 10.0-1000.0 mg l-1, respectively. The detection limit for MPA and MPM was obtained as 0.3 mg l-1 and 1.1 mg l-1, respectively. Satisfactory results were obtained by the combination of spectrophotometric method and chemometrics techniques. The method was successfully applied to the simultaneous determination of MPM and MPA in serum sample and the results were comparable with HPLC method.


1. G.Bahrami, B.Mohammadi, Clinica Chimica Acta 370 (2006) 185.
2. A.C. Allison , E.M. Eugui, Clin Transplant 7(1993) 96.
3. R.E. Morris, J. Heart and Lung Transplantation 12(1993) S275.
4. C. J.Young, H.W. Sollinger, Transplant Proc. 26(1994) 3144.
5. K.Na-Bangchang, O.Supasyndh, T.Supaporn, V.Banmairuroi, J. Karbwang, J. Chromatogr. B 738(2000)169.
6. A. Elbarbry Fawzy, S. Shoker Ahmed, J. Chromatogr. B 859(2001) 276.
7. X.Delavenne, L. Juthier, B.Pons, C.Mariat, T.Basset, Clin. Chim. Acta 412 (2011) 59.
8. J.Kuhn, C.GŁtting, K.Kleesiek, Talanta 80 (2010) 1894.
9. G. Absalan, M. Solemani, Anal. Sci. 20 (2004) 879.
10. T. Irene, K. Martin, J. Chromatogr. B 681(1996) 347.
11. N. Sugioka, H. Odani, T. Ohta, H. Kishimoto, T. Yasumura, K. Takada, J. Chromatogr. B 654 (1994) 249.
12. U.D. Renner, C. Thiede, M. Bornhauser, G. Ehninger, H.M. Thiede, Anal. Chem. 73 (2001) 41.
13. I. Tsina, M. Kaloostian, R. Lee, T. Tarnowski, B. Wong, J. Chromatogr. B 681 (1996) 347.
14. M. Plätzer, K. Jahn, J. Wohlrab, R.H.H. Neubert, J. Chromatogr. B 755 (2001) 355.
15. S. Verma, H. Gupta, O. Alam, P. Mullick, N. Siddiqui, S.A. Khan, J. Appl. Spect. 76 (2009) 876.
16. V.P. Tripodi, S.E. Lucangioli, C.L. Barbara, V.G. Rodríguez, C.N. Carducci, Chromatographia 54 (2001) 93.
17. T. Madrakian, M. Soleimani, A.Afkhami, Materials Science and Engineering C 42 (2014) 38.
18. E. Schuetz, M. Shipkova, V.W. Armstrong, M. Oellerich, Clin. Chem. 45 (1999) 419.
19. B. Eric, T. Hastie, P. Debashis, R. Tibshirani, J. Amer. Statis. Assoc. 101 (2006) 119.
20. R. Roman, N. Krämer, Lecture Notes in Computer Science 3940 (2006) 34.
21. D.M. Haaland, E.V. Thomas, Anal. Chem. 60 (1988) 1193.
22. D.M. Haaland, E.V. Thomas, Anal. Chem. 60(1988) 1202.
23. E. Dinc, D. Baleanu, F. Onur, J. Pharm. Biomed. Anal. 26(2001) 949.
24. N. H. Al- shaalan, J. Saudi Chem. Soc. 14(2010)15.